Rizky Muzakki posted 7 months ago
What blank should i use in Bradford assay?
I'm doing a Bradford assay but currently I'm still confused as to what blank i should use for my sample, which is the supernatant of PAO1 culture grown in AB minimal medium. For the standard, i use serially diluted BSA in dH2O, so the blanks should be a mixture of Bradford reagent and dH2O. But for the sample's blank, what should i use? Is it correct if I use the dH2O blank as well, but also add another sample of fresh AB medium, then subtract the absorption of fresh AB medium from the absorption of PAO1 culture's supernatant?

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